FastQCFastQC Report
Mon 6 Jun 2016
ERR1378338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978817
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCATTATCTCGTATGCC28870.2949478809624271Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT27210.2779886332174451Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACCATTATCTCGTATGCCGTC22930.2342623799954435Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2800.035.35276845
ATGCCGT2950.033.55517244
GATCGGA4450.033.36665726
AGATCGG4500.032.9959225
ATCGGAA4650.031.44772127
TATGCCG3150.030.71048743
CGCAATT300.005150004329.99628835
TCGGAAG4900.029.84324628
GAGATCG5150.028.83138324
CCGAGAT5250.028.71073222
CGAGATC5350.027.75357423
AATGCCG10400.023.79513418
ATGCCGA10400.023.79513419
TGCCGAG10900.022.70361120
GACCGAC5800.022.49721529
ACCGACC5900.022.11590830
AGACCGA5900.022.11590828
GCCGAGA11250.021.99727621
CCGACCA5950.021.9300631
ATCTCGT6000.021.74730940
Produced by FastQC (version 0.11.5)