FastQCFastQC Report
Mon 6 Jun 2016
ERR1378337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624627
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACATTATCTCGTATGCC21820.3493284792364083Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT21420.3429246574355575Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCACATTATCTCGTATGCCGTC18360.2939354206590493Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1850.038.91400544
TATGCCG1950.036.9184143
TGCCGTC2000.035.99545345
ATCGGAA3000.032.9958327
GATCGGA3100.031.9314526
AGATCGG3250.030.45769125
TCGGAAG3300.029.9962128
CCGTTCA406.167522E-428.12144739
GAGATCG3850.026.29538224
CCGAGAT4050.024.99684122
CGAGATC4100.024.69200323
CGTATGC4000.024.18444444
AGACCGC4150.023.31030828
TCGTATG4150.023.31030843
GTATGCC4300.023.02034845
TCTCGTA4050.022.774941
CCGCACA4250.022.76183131
GAGACCG4350.022.75574527
CTCGTAT4100.022.4971642
GACCGCA4300.022.49715829
Produced by FastQC (version 0.11.5)