FastQCFastQC Report
Mon 6 Jun 2016
ERR1378336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694913
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAATTATCTCGTATGCC31980.46020149284874506Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT31100.4475380371355839Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAATTATCTCGTATGCCGTC26010.37429145806741276Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.041.5988543
TGCCGTC2750.039.2680845
ATGCCGT2850.037.8902544
AGATCGG4300.034.00760325
TCGGAAG4700.033.5066728
GATCGGA4450.033.36683726
ATCGGAA4650.031.93170527
TGAACCG300.005148852229.99645210
ACGGATA300.005148852229.99645231
TCGTATA751.7025741E-929.996459
CGAGATC5100.028.67307923
CCGAGAT5250.028.28236622
GAGATCG5300.027.59107624
AATGCCG10750.024.48547418
ATGCCGA10750.024.48547419
TGCCGAG10850.024.4671520
TTCGTAT952.114939E-823.6814088
GACCGCC6000.023.2472529
TATCTCG5950.023.06449939
AGACCGC5950.023.06449928
Produced by FastQC (version 0.11.5)