FastQCFastQC Report
Mon 6 Jun 2016
ERR1378335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007003
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT49010.48669169803863543Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTGTATCTCGTATGCC36270.3601776757368151Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTGTATCTCGTATGCCGTC35430.35183609184878295Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.038.63691343
TGCCGTC4700.038.29351845
ATGCCGT4900.036.2713944
GATCGGA7700.031.55485526
ATCGGAA7700.031.55485527
AGATCGG7900.030.75599925
TCGGAAG8200.030.17949728
CCGAGAT9000.027.49687622
GAGATCG9100.026.94748724
CGAGATC9550.025.91328423
AATGCCG18200.023.73356618
TGCCGAG18200.023.60995320
ATGCCGA18300.023.60387219
GCCGAGA19000.022.26062821
GAATGCC20000.022.04749517
GGAATGC20800.021.09135416
CTCGTAT8550.020.78711139
TCTCGTA8550.020.78711138
CGTATGC8650.020.54679941
CGGAAGA12200.020.46898729
Produced by FastQC (version 0.11.5)