FastQCFastQC Report
Mon 6 Jun 2016
ERR1378334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645993
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39600.6130097384956184Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCC37030.5732260256690088Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCCGTC30300.46904533021255645Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3600.038.1212643
ATGCCGT3750.036.59641344
TGCCGTC3900.035.18885845
GATCGGA6100.034.29991526
AGATCGG6100.033.931125
TCGGAAG6250.033.8366828
ATCGGAA6150.033.65523527
CCGAGAT6600.032.3831922
CGAGATC6700.031.22828123
GAGATCG6950.030.10496324
AATGCCG14000.025.06897218
TGAACCG450.001226673824.9975510
ATGCCGA14050.024.81963219
TGCCGAG14400.024.3726120
GCCGAGA14150.024.32623721
GAATGCC14700.023.87521217
CTCGTAT7450.023.25275442
GACCGTT7400.023.1058429
TATCTCG7550.022.94476939
ACCGTTG7500.022.79776630