FastQCFastQC Report
Mon 6 Jun 2016
ERR1378333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535747
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT42810.7990712033851799Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCTGAATCTCGTATGCCGTC37480.6995839454070671Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCTGAATCTCGTATGCC32340.6036431375257352Illumina Paired End PCR Primer 2 (97% over 37bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8120.15156407782031445Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.041.08218443
ATGCCGT4800.039.37042644
TGCCGTC4950.038.17738345
GTCGGAA301.1398876E-437.49564427
ATCGGAA6000.034.4959927
AGATCGG6100.034.29929425
GATCGGA6050.034.21090326
TCGGAAG6600.032.72347328
CCGAGAT6500.032.53468322
GAGATCG6650.031.8008224
CGAGATC6750.031.32969323
CAGACCG300.005147757529.99651512
GGTCGGA406.1659986E-428.12173526
TGCCGAG15350.026.67442520
AATGCCG15550.026.62070318
ATGCCGA15700.026.36636419
GCCGAGA15600.025.67009521
GAATGCC16200.025.55258817
GGAATGC16650.024.72685616
AGGAATG17850.023.19058215
Produced by FastQC (version 0.11.5)