FastQCFastQC Report
Mon 6 Jun 2016
ERR1378332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308788
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTAGTATCTCGTATGCC39500.30180594565353597Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39140.29905530918682016Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTAGTATCTCGTATGCCGTC32120.24541789808586265Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3000.038.2456843
ATGCCGT3300.034.76880344
TGCCGTC3500.032.78201345
ATCGGAA5350.029.85644127
GATCGGA5450.029.72141326
AGATCGG5800.027.9278825
CCGCTTA7900.026.19957231
CGCTTAG7900.026.19957232
TCGGAAG6600.025.56529428
CGTATGC8200.025.5154144
ACCGCTT8200.025.2410530
CTCGTAT8350.025.0570542
TCTCGTA8350.025.0570541
TCGTATG8500.024.87954343
AGACCGC8350.024.7876228
GTATGCC8550.024.73404945
TATCTCG8500.024.61486639
GACCGCT8600.024.06704929
GAGACCG8800.024.03137627
CCGAGAT7000.023.7830322