FastQCFastQC Report
Mon 6 Jun 2016
ERR1378331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929203
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCC31480.3387849587226903Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT27740.2985354115300962Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCCGTC25930.27905635259464295Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG4350.034.13368625
GATCGGA4350.034.13368626
TATGCCG3200.033.745843
ATGCCGT3150.033.56725344
ATCGGAA4500.032.995927
AAGCGCA300.005149888329.99626710
TGCCGTC3550.029.78502845
TCGGAAG5150.029.70504428
CCGAGAT5150.028.83136622
GAGATCG5350.027.7535624
CGAGATC5650.026.27991923
AATGCCG11150.023.00162318
ATGCCGA11400.022.49720419
TGCCGAG11500.022.30157520
GAATGCC12300.021.0339717
TATCTCG6050.020.82385439
GCCGAGA12250.020.7525221
GACCGTT6150.020.11944829
ACCGTTC6150.020.11944830
CGTTCAG6300.019.99751332
Produced by FastQC (version 0.11.5)