FastQCFastQC Report
Mon 6 Jun 2016
ERR1378330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492113
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTCTATCTCGTATGCC37380.7595816408020108Illumina Paired End PCR Primer 2 (97% over 38bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT37080.7534854799609033Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTCTATCTCGTATGCCGTC33750.6858180946246086Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC5960.12111039537667162Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4150.039.03376444
TATGCCG4250.038.64470343
TGCCGTC4450.036.4022845
ATCGGAA4700.030.6364327
GATCGGA4800.029.9981726
AGATCGG4950.029.54365525
CCGAGAT5200.029.42128222
GAGATCG5100.029.11587124
TCGGAAG5100.028.6747228
CGAGATC5400.027.49832223
CCGAACT9050.023.3687431
GACCGAA9200.022.9877329
ACCGAAC9200.022.9877330
TATCTCG9250.022.8634739
CTCGTAT9300.022.7405542
GTATGCC9300.022.7405545
GAGACCG9250.022.62024127
CGTATGC9350.022.61894244
TCTCGTA9350.022.61894241
ATCTCGT9450.022.37958740
Produced by FastQC (version 0.11.5)