FastQCFastQC Report
Mon 6 Jun 2016
ERR1378328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098570
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT28160.2563332332031641Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCCAATCTCGTATGCC27000.2457740517217838Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCCAATCTCGTATGCCGTC22500.20481170976815316Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2100.037.4960245
TATGCCG2100.036.42470643
ATGCCGT2100.036.42470644
ATCGGAA3250.031.84277227
GATCGGA3400.030.43794426
AGATCGG3600.028.74694425
ACGCCCG903.965397E-1027.49707812
CGTATCC5100.026.90890732
CTCGTAT5250.026.1400842
GACCGTA5300.025.89347529
TCTCGTA5300.025.89347541
TCGGAAG4000.025.87225228
ACCGTAT5400.025.41396730
AGACCGT5450.025.18081328
ATCTCGT5500.024.95189540
CCGTATC5500.024.95189531
GAGACCG5600.024.9080727
CGTATGC5550.024.72710244
TCGTATG5550.024.72710243
GAGATCG4400.023.5202324
Produced by FastQC (version 0.11.5)