FastQCFastQC Report
Mon 6 Jun 2016
ERR1378326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645732
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTACAATCTCGTATGCCGTC13970.21634362243159702Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13630.21107828015337632Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTACAATCTCGTATGCC9730.1506817069620214Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1350.029.99690243
ATGCCGT1400.028.92558744
TGCCGTC1650.024.54292145
AGATCGG1950.023.07454125
CGCTTAC2400.022.49767932
CCGCTTA2350.022.01900331
ATCTCGT2450.021.12026840
ATCGGAA2250.020.99783127
GAACCGC654.2384127E-420.76708611
AATGCCG4450.020.72819718
CGTATGC2500.020.69786344
TCTCGTA2500.020.69786341
GATCGGA2200.020.45243326
TCGGAAG2100.020.35504228
TCGTATG2550.020.29202543
GACCGCT2700.019.99793429
CTCGTAT2600.019.9017942
AGACCGC2600.019.9017928
ACCGCTT2650.019.52628730
ACCGCTC706.983784E-419.28372413
Produced by FastQC (version 0.11.5)