FastQCFastQC Report
Mon 6 Jun 2016
ERR1378325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094825
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTACCATCTCGTATGCC56570.5167035827643688Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT47670.4354120521544539Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTACCATCTCGTATGCCGTC40800.3726622976274747Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5250.038.13844343
ATGCCGT5400.036.66242244
TGCCGTC5500.036.4048845
GATCGGA6800.031.43018726
AGATCGG6900.031.30072825
ATCGGAA7050.030.31564127
ACGGGGC300.005150066729.99652935
TCGGAAG7750.029.02889828
CGAGATC7650.028.5261123
CCGAGAT7700.028.04870222
CCGCTCT654.8779657E-727.68910414
GAGATCG7800.027.68910424
CCGATTA12200.024.5258531
GACCGAT12200.024.5258529
CGTATGC12250.024.24209244
GAACCGC651.5250313E-524.22796611
ACCGATT12400.024.13027230
TCGTATG12350.024.04580143
ATCTCGT12450.023.85266140
CGATTAC12550.023.84186432
Produced by FastQC (version 0.11.5)