FastQCFastQC Report
Mon 6 Jun 2016
ERR1378322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885473
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATTACATCTCGTATGCCGTC42900.48448682229723544Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT41060.4637069679143238Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATTACATCTCGTATGCC38430.43400532822570537Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5500.041.31465543
ATGCCGT5800.039.17769244
TGCCGTC6150.036.94806745
GATCGGA5100.036.17338626
AGATCGG5050.036.08603325
ATCGGAA5200.035.4777427
TCGGAAG5500.034.7697628
CGAGATC5950.030.62763623
CCGAGAT6050.030.49326522
GATTCGG300.005148775429.9974412
GAGATCG6400.028.47413324
AATGCCG15050.027.50595918
ATGCCGA15050.027.50595919
TGCCGAG15200.027.08650620
GAATGCC16150.025.91110217
GCCGAGA15900.025.61102121
CGTATGC9850.023.29750641
GACCGCA9700.023.19389726
CTCGTAT9800.023.18679839
TCGTATG9800.023.18679840
Produced by FastQC (version 0.11.5)