FastQCFastQC Report
Mon 6 Jun 2016
ERR1378321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080596
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50870.47075872944190056Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTACATCTCGTATGCCGTC46870.4337421200892841Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTACATCTCGTATGCC46190.42744929649933927Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5550.041.34650443
ATGCCGT5650.040.61470844
TGCCGTC5850.039.2261745
GATCGGA5950.032.89530626
AGATCGG6000.032.62117425
ATCGGAA6000.032.62117427
TCGGAAG7350.029.69039728
GAGATCG7150.027.37441324
AATGCCG16650.026.618518
ATGCCGA16700.026.53880519
CCGAGAT7650.026.46748522
CGAGATC7650.026.17340323
TGCCGAG17050.025.9940220
GAATGCC18650.023.884617
GCCGAGA17750.023.82819421
GGAATGC19500.022.95884716
TCTCGTA10100.022.49736238
GTCGGAA903.290279E-722.49736227
CGTATGC10300.022.27894241
CTCGTAT10300.022.27894239
Produced by FastQC (version 0.11.5)