FastQCFastQC Report
Mon 6 Jun 2016
ERR1378320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025539
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTAAATCTCGTATGCC40950.39930222058839304Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT40620.3960844004957393Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTAAATCTCGTATGCCGTC34320.33465328963598656Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4400.036.81239744
TGCCGTC4400.036.81239745
TATGCCG4500.036.49426743
ATCGGAA6050.034.95318627
TCGGAAG6250.033.83468228
GATCGGA6300.033.56615426
AGATCGG6500.032.87944825
CGAGATC7700.028.0475423
GAGATCG7700.027.75537924
CCGAGAT7850.027.511622
ATGCCGA15300.024.40792819
AATGCCG15350.024.32842318
TGCCGAG15650.023.86206420
CGCTCTA8700.021.97930532
GCCGAGA17150.021.9061821
TCTCGTA9000.021.49662241
CGTATGC8900.021.48538844
ACCGCTC8950.021.36535830
GAATGCC17500.021.33950417
GACCGCT9100.021.26039529
Produced by FastQC (version 0.11.5)