FastQCFastQC Report
Mon 6 Jun 2016
ERR1378319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930770
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCCTAAATCTCGTATGCC48240.518280563404493Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT48140.5172061841271206Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCCTAAATCTCGTATGCCGTC38410.41266908043877654Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4200.041.78217344
TGCCGTC4400.039.8829845
TATGCCG4700.037.8159443
AGATCGG6250.033.4771625
GATCGGA6500.032.1895826
ATCGGAA6600.031.3609827
TCGGAAG7150.030.20723228
CGAGATC7300.028.66195323
CCGAGAT7350.028.46697622
GAGATCG7350.028.46697624
CGACGAC406.168314E-428.12261643
TGCCGAG16750.023.5054720
AATGCCG16800.023.43551418
ATGCCGA16950.023.36085119
GACCGTC9450.022.37905529
ACCGTCC9450.022.37905530
CGTCCTA9500.022.26126932
CGTATGC9650.022.14838244
TCGTATG9650.022.14838243
TCTCGTA9550.022.14472241
Produced by FastQC (version 0.11.5)