FastQCFastQC Report
Mon 6 Jun 2016
ERR1378317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522691
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGCATATCTCGTATGCC33840.647418838281126Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT27990.5354980284718888Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGCATATCTCGTATGCCGTC26810.5129225488864358Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3250.039.45809645
ATGCCGT3300.038.8602544
TATGCCG3450.037.17067343
GATCGGA3850.031.55569326
TCGGAAG3950.030.75681128
ATCGGAA3950.030.75681127
AGATCGG3950.029.6176725
CCGAGAT4300.027.2069322
GAGATCG4600.025.43256624
CGAGATC4900.023.87547123
CGTTGCA7350.023.26327732
TCTCGTA7350.023.26327741
CGTATGC7400.023.10609444
AGACCGT7350.022.95718228
CTCGTAT7450.022.9510242
GTATGCC7450.022.9510245
TCGTATG7450.022.9510243
GACCGTT7400.022.80206729
CCGTTGC7500.022.79801431
ACCGTTG7550.022.64703230
Produced by FastQC (version 0.11.5)