FastQCFastQC Report
Mon 6 Jun 2016
ERR1378316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931298
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACACATCTCGTATGCC35440.38054414376493884Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT34170.3669072627665903Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACACATCTCGTATGCCGTC27410.29432040012971145Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3450.035.2148743
ATGCCGT3550.034.222944
TGCCGTC3600.033.7475845
ATCGGAA4550.033.12949827
GATCGGA4700.032.5508626
AGATCGG4800.031.87271925
TCGGAAG5150.030.58033628
GAGATCG5450.028.48419824
CGAGATC5850.026.53656223
CCGAGAT6100.025.81782522
ATGCCGA11900.024.01088519
TGCCGAG12000.023.62330820
AATGCCG12150.023.33166518
GCCGAGA12950.021.19539521
GACCGTT6150.020.85216529
AGACCGT6200.020.68400428
CCGTTAC6200.020.68400431
GAGACCG6350.020.54971127
CGTTACA6300.020.35568632
GAATGCC14250.020.2090817
Produced by FastQC (version 0.11.5)