FastQCFastQC Report
Mon 6 Jun 2016
ERR1378315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010159
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCTAATATCTCGTATGCC49520.4902198564780396Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43140.4270614824002954Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCTAATATCTCGTATGCCGTC40900.40488675545136954Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5150.040.19028543
ATGCCGT5250.038.99622344
TGCCGTC5500.037.6327245
ATCGGAA5450.030.96030427
GATCGGA5700.030.78649326
AGATCGG5700.029.99709525
TCGGAAG6100.028.76770628
CCGAGAT6650.026.38842422
CGAGATC6650.026.38842423
GAGATCG7050.024.89120724
AATGCCG17350.022.56265618
ATGCCGA17800.022.11864719
TGCCGAG17500.022.11214620
TCTCGTA10800.021.87288341
CGTATGC10750.021.76533544
CTCGTAT10750.021.76533542
TCGTATG10800.021.6645743
TATCTCG10900.021.4658139
CGCCTAA10950.021.36779432
GTATGCC11000.021.27066845
Produced by FastQC (version 0.11.5)