FastQCFastQC Report
Mon 6 Jun 2016
ERR1378312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116335
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCAATATCTCGTATGCC37310.33421867091867585Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32460.2907729310645998Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCAATATCTCGTATGCCGTC30970.2774256831506671Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.038.71846443
ATGCCGT4600.036.19334444
TGCCGTC4600.036.19334445
ATCGGAA4200.031.0694527
TCGGAAG4600.029.83505428
AGATCGG4450.029.82956125
GATCGGA4550.029.6684426
AATGCCG12950.023.97530618
ATGCCGA13050.023.9639919
TGCCGAG13050.023.79158620
CCGAGAT5750.023.47676722
CGAGATC5900.023.2612323
CTCGTAT7900.021.64419242
GAGATCG6350.021.25848624
CGTATGC8050.021.24088344
TCTCGTA8150.020.98025941
TCGTATG8200.020.8523343
GCCGAGA14500.020.79177921
CGCTCAA8300.020.60109732
ATCTCGT8350.020.47773737
Produced by FastQC (version 0.11.5)