FastQCFastQC Report
Mon 6 Jun 2016
ERR1378311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013612
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGATTAATCTCGTATGCC43160.4258039565435295Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGATTAATCTCGTATGCCGTC38360.3784485582254354Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT37210.36710299404505864Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4550.040.54473543
TGCCGTC4600.040.10403445
ATGCCGT4900.037.64868544
GCGTCAA300.005149900429.99651515
CGATAGA300.005149900429.99651523
ATCGGAA4100.029.08198727
GATCGGA4250.028.58491326
AGATCGG4400.026.5878225
TCGGAAG4900.024.33390628
CGAGATC4900.023.87477523
GGTGCGA500.002265955822.49738713
CGGAAGA9450.022.1632441
AATGCCG14100.021.38049318
GACCGAG9450.021.18801329
ACCGAGA9250.021.15970230
ATGCCGA14250.021.15543719
CTCGTAT9500.021.07649842
CGTATGC9550.020.96614844
TGCCGAG14450.020.86262720
AGACCGA9550.020.73057428
Produced by FastQC (version 0.11.5)