FastQCFastQC Report
Mon 6 Jun 2016
ERR1378308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995465
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACATAATCTCGTATGCC27080.27203367270572043Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT23030.2313491684790525Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACATAATCTCGTATGCCGTC19410.1949842535900308Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2750.037.63199643
TGCCGTC2750.036.81390845
ATGCCGT2900.034.90974444
AGATCGG3600.034.3710125
GATCGGA3600.034.3710126
TCGGAAG3950.031.89503128
ATCGGAA4000.030.93390827
CCGAGAT4400.028.12173722
ACCGCTC406.169747E-428.12173513
CGAGATC4600.027.38812423
GAGATCG4700.026.3267324
ATGCCGA9100.024.96962719
TGCCGAG9100.024.96962720
AATGCCG9450.024.28289418
CCGCTCT500.002265926222.49738914
GCCGAGA10100.022.27464321
GAATGCC10600.021.8606717
CGTATGC5450.020.63980544
ACCGAAC5450.020.22700930
GCGGTTC11350.020.0358055
Produced by FastQC (version 0.11.5)