FastQCFastQC Report
Mon 6 Jun 2016
ERR1378307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773792
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT551.12737325E-732.72572342
GACCGTT602.4168912E-730.00051929
CGTATGC654.86918E-727.69278744
AGCGGTT654.86918E-727.6927877
CGTTTCG654.86918E-727.69278732
TTTCGAA654.871945E-727.69099634
CGAATCT654.871945E-727.69099637
TCGTATG654.871945E-727.69099643
TTCGAAT654.871945E-727.69099635
TCGAATC654.871945E-727.69099636
GCGGTTC709.3047856E-725.7130688
GTTTCGA851.8928586E-723.822433
GAGCGGT802.9705589E-622.5018426
AGACCGT802.972225E-622.50038728
TCTCGTA802.9738949E-622.49893441
GAGACCG855.0279505E-621.17683427
CGGTTCA855.0307663E-621.1754679
AATGCCG908.248906E-619.99905221
AAGAGCG1002.0415186E-518.00384
AGAGCGG1002.0437905E-518.0014745
Produced by FastQC (version 0.11.5)