FastQCFastQC Report
Mon 6 Jun 2016
ERR1378306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989382
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA450.001226474324.9998641
ACGCCCG551.3685363E-424.54407512
GACCGGA500.002264462922.49987229
ACCGGAA550.00393628520.4544330
CTCGTAT550.00393628520.4544342
CGGCTAA1051.445931E-619.2846317
AGACCGG600.006510082618.74989528
TCGTATG600.006510082618.74989543
CTTCGTA851.2047445E-418.528377
ATCTCGT1002.0462661E-517.99989940
GTGCGAT750.001110493117.9989914
CCGAGAC901.865426E-417.49990325
CCGGCTA1053.113215E-517.14189316
CGGAAAG800.001710682116.87490532
GGTGCGA800.001711260616.87405213
CGGTTCA900.003754831914.9991579
CACGCCC1402.2305263E-514.46347311
TGGTGCG950.00537731714.20972812
GCCCGGC2254.8967195E-913.99921414
CGCCCGG1301.9266183E-413.84537613
Produced by FastQC (version 0.11.5)