FastQCFastQC Report
Mon 6 Jun 2016
ERR1378305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922428
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT552.744855E-936.8213776
GGTCACG300.00514728329.99935239
GACCGTT453.490621E-529.9993529
AGCGGTT554.16271E-628.6372977
CGTATGC551.3681178E-424.54492444
CTCGTAT551.3681178E-424.54492442
AGAGCGG851.8912215E-723.8255945
CGGAAGA702.6993033E-522.5031721
GCGGTTC602.466453E-422.5007328
AAGAGCG1056.0954335E-821.4315914
ACGCCCG550.003936451420.45410312
CCGAGAC807.562192E-519.68707325
AGACCGT750.001110159217.99960928
AATGCCG901.8654826E-417.4996221
GAGACCG800.001710747816.87463427
CGCCCGG800.001710747816.87463413
AATCTCG850.002565021415.88200939
ATGCCGA850.002565021415.88200922
ATCTCGT900.003753714514.99967540
CGGTTCA900.003753714514.9996759
Produced by FastQC (version 0.11.5)