FastQCFastQC Report
Mon 6 Jun 2016
ERR1378303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937519
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT356.248174E-638.57042329
ATCGGTG250.002107180735.9990623
AGACCGG458.699426E-734.9990928
ACCGGTT453.4907862E-529.99921830
TCTCGTA453.4907862E-529.99921841
GCGTCAG300.005147435729.99921822
CGCCAAT300.005147435729.99921819
TCGTATG507.1582865E-526.99929643
CGTATGC608.1910985E-626.24931544
GCGGTTC654.238175E-420.7686888
CGGTTTA654.238175E-420.76868832
GATGTCG550.003936620.45401225
AGAGCGG807.559515E-519.6880365
ATGCCGA807.562614E-519.68698722
AGCGGTT706.98338E-419.2852127
CGATATT600.00651060118.74951211
AAGAGCG851.2019205E-418.5338734
CGGAAGA750.001108225118.0043321
GAGCGGT750.001109813118.000496
CTCGTAT750.001110210417.99952942
Produced by FastQC (version 0.11.5)