FastQCFastQC Report
Mon 6 Jun 2016
ERR1378302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856477
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA401.5593982E-533.7522341
GACCGTT453.4896308E-530.00023329
CGTATGC507.153083E-527.00178744
CTCGTAT507.153083E-527.00178742
GAGACCG551.3677328E-424.54564527
ATCTTCG855.0293984E-621.1766365
CGGTTCA654.2384613E-420.768189
TGAGCCG1703.6379788E-1219.85425645
ACCGTTT706.981112E-419.28586630
CGTATAA1102.255705E-618.4081610
CGGAAGA750.001107245118.0064471
GAGCGGT750.001109851518.000146
AGACCGT750.001109851518.0001428
CTTCGTA1002.0465366E-517.9990887
TCGTATG800.001709607716.87611643
AGAGCGG800.001710275816.8751325
GCGGTTC800.00171094416.8741468
TCGCTTG1257.6252763E-616.1991815
TCGTATA1257.6252763E-616.199189
ATCGCTT1552.0654079E-715.96693514
Produced by FastQC (version 0.11.5)