FastQCFastQC Report
Mon 6 Jun 2016
ERR1378301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980785
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG550.00393742420.45338627
CGGCTAA1104.638649E-516.36270917
CCGGCTA1108.590444E-414.31737116
CGTTTCC2155.944039E-712.5663971
CCACCGC1801.6918677E-512.50056657
ATCTTCG1506.445026E-412.0005445
TCCAACG2004.9720238E-511.250515
GCCACCG1802.3424567E-411.2505096
AGCTATC1600.001108164211.24936222
CCAACGA2108.166592E-510.7147716
CGAAATC1700.001837894610.58763510
CCCGGCT1950.00572158689.23024615
GGCCGAG2300.00222626588.80384910
GCCGAGG2300.00222626588.80384944
CCTAACT3351.2338109E-58.73084840
CAACGAA2606.99114E-48.6542397
AGGCGGA2350.00270570678.61653230
AGGCCGA2350.00270570678.6165329
ACTAGAC3401.4872519E-58.60245419
CTAGACA5005.452439E-77.64956720
Produced by FastQC (version 0.11.5)