FastQCFastQC Report
Mon 6 Jun 2016
ERR1378300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733430
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA654.23262E-420.7718456
ACGAAGG952.8193038E-416.577649
CGTTTCC1754.8390575E-815.4326171
CCTTCGT1056.0211745E-415.0039331
CCAACGA1654.1877865E-715.0018866
ACGGGAT900.003754163614.99881713
ACCACGC1351.581179E-514.9988179
CGGGATT950.005376368814.20940714
CGAAGGC1150.001201765713.69457310
TCGCTCT1000.00753791513.5026192
CCGGCTA1000.00755152713.49893716
CGGCTAA1000.00755152713.49893717
TCCAACG1851.5233309E-613.3800615
CAACGAA1851.5256683E-613.3782377
CTTCGTT1200.001652996213.1275462
AACGGGA1250.002251505912.59900812
TCGTTGG1454.836906E-412.4162014
CGTTGGA1454.839686E-412.4153555
CCACGCC1651.0426722E-412.27176110
GCACTCC2401.7107777E-712.18654116
Produced by FastQC (version 0.11.5)