FastQCFastQC Report
Mon 6 Jun 2016
ERR1378299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095897
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC453.4919787E-529.99838844
ATCGTAG300.005148508629.99838633
GAGCGGT554.160531E-628.6400536
CTCGTAT602.4683052E-422.49878942
GGGCGAC754.6042886E-520.9988728
GCGGTTC807.560405E-519.6882388
GACCGAG706.985711E-419.28467829
TCTCGTA706.985711E-419.28467841
AGACCGA706.985711E-419.28467828
CCGAGAC706.985711E-419.28467825
TTGCGCC600.0065054718.7524156
ATCTCGT851.2048723E-418.52841640
ACCGAGG851.2048723E-418.52841630
CGGTTCA1002.0474394E-517.9990339
TCGTATG750.00111057917.99903343
AGAGCGA1153.4501372E-617.6077535
AGAGCGG800.001709302716.8771745
AGCGGTT800.001710346816.8756337
CGAGGAA800.001711391516.87409232
ATGCCGA800.001711391516.87409222
Produced by FastQC (version 0.11.5)