FastQCFastQC Report
Mon 6 Jun 2016
ERR1378298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767580
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG300.00514587730.00039143
TTACCGT300.005147516729.99843622
CACCACG2005.0931703E-1116.874128
ACCACGC1907.9871825E-915.3939359
CACGCCC1402.229153E-514.46353211
CCGGCTA1251.3801176E-414.3992516
CGGCTAA1150.001201613313.69493917
AACCCGG1504.268977E-513.49929722
CCACGCC1902.059971E-613.02563710
TGTAGAC1403.6036258E-412.85731140
GAACCCG1403.6056092E-412.85647421
TCCAACG1454.8447403E-412.4139565
CCCGCCA1506.429858E-412.0032843
TCTCGAT1558.490422E-411.6122989
GTGGTTC1650.001432365710.90852321
CCAACGA1450.006820406810.8622116
ACCCGGG1450.0068237110.86150323
TGAGCCG1700.001836044710.58837345
GAGTAGC2302.0481698E-49.78273643
GCTAGAC1900.00461743459.4731918
Produced by FastQC (version 0.11.5)