FastQCFastQC Report
Mon 6 Jun 2016
ERR1378297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115222
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG300.005148020629.99901623
AGACCGT550.00393721520.45387328
TCGTATG550.00393721520.45387343
GCGGTTC750.00111010618.0002168
CCTTCGT1552.0571679E-715.9736621
CCGAGAC850.002565662815.88183125
ACGGGAT1502.5742138E-614.99950713
ATGCCGA950.005377054714.21005922
TCGTTGG2408.0035534E-1014.065194
CGTTGGA2306.661139E-913.6964315
AGCGGTT1000.007550235413.5001617
CGGTTCA1000.007552474713.4995569
TTCGTTG2551.9845174E-913.23842053
CGAGGCA1403.5977567E-412.8607562
CGGGATT1506.4504857E-411.99960614
TGCCCGG1751.8049226E-411.57104913
AAACGGG2656.186074E-711.03737311
CGCCACC2251.35827395E-511.0006255
CTTCGTT2301.7282333E-510.7638942
GGCTAAT4850.010.66975218
Produced by FastQC (version 0.11.5)