FastQCFastQC Report
Mon 6 Jun 2016
ERR1378296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156652
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT801.0004442E-1030.9365842
GTCGATG300.00514802229.99910735
GACCGTA852.0372681E-1029.1180429
AGCGGTT952.3646862E-1128.4214367
CGGAAGA903.947207E-1027.5098861
TCTCGTA903.965397E-1027.49918241
CGTATGC1004.5474735E-1126.99919544
ATGCCGA1004.5474735E-1126.99919522
TCGTATG957.421477E-1026.05185743
GAGACCG1001.3442332E-924.75033427
AGACCGT1001.3442332E-924.75033428
CGTAAAC1001.3442332E-924.75033432
GCGGTTC1101.5279511E-1024.5457868
AAGAGCG1152.6557245E-1023.4806064
CGGTTCA1204.5110937E-1022.499339
AATGCCG1204.5110937E-1022.4993321
GAGCGGT1301.2278178E-920.7695086
CCGAGAC1301.2278178E-920.7686125
TGCCGAG1301.2278178E-920.7686123
CCGGCTA1351.9645086E-919.99940516
Produced by FastQC (version 0.11.5)