FastQCFastQC Report
Mon 6 Jun 2016
ERR1378294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949117
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTA955.531001E-721.3152416
CGGCTAA951.3157296E-518.9468817
AATATCG600.00650075218.7544572
ACGGGTT600.00651064718.74951713
ACCACGC1305.7815305E-717.3072459
CGCCCGG850.002565163215.88194513
CCTCGTG1301.1062555E-515.57652123
CCTTCGT1451.8014689E-615.5225661
GCCCGGC1754.861431E-815.42817414
TCGTTGG2051.3114914E-915.3686974
GCGTGAT1056.0366665E-414.99961314
CGTTGGA1705.8827027E-714.55921655
TTCGTTG2102.9791408E-813.9311483
CGGGATT1150.001201741413.695314
CGCCCGC1000.007532628313.5046331
CCACGCC1504.2700034E-513.49965310
ACCTCGT1352.6517606E-413.3329922
ACTAGAC3900.012.69198119
TCGTGAT1607.8211786E-512.65592425
CCAACGA1250.002250562712.6003396
Produced by FastQC (version 0.11.5)