FastQCFastQC Report
Mon 6 Jun 2016
ERR1378293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190093
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA552.750312E-936.81821432
TACGAGC250.002106693836.001554
TCGTATG651.4037141E-831.15387743
CGTACTT300.005147308630.00002530
GAGCGGT755.639413E-827.0000276
CGTATGC801.0538497E-725.31252344
ATCTCGT851.8937499E-723.82355140
CTCGTAT903.287114E-722.50002142
CGGTTCA1003.6461643E-822.4990779
ACCGCAT1056.1081664E-821.4285930
AGACCGC1056.1081664E-821.4285928
TTCGGAC550.003935639320.4554254
GACCGCA1109.9847966E-820.45456529
TCTCGTA1009.055384E-720.25002141
AGCGGTT1251.7682396E-819.7991857
GCGGTTC1051.4462257E-619.2849228
CCGCATA1102.2560962E-618.40910731
ATGCCGA1305.7829493E-717.30770922
CGGAAGA1301.10380515E-515.5808651
AGAGCGG1301.1064463E-515.5769395
Produced by FastQC (version 0.11.5)