FastQCFastQC Report
Mon 6 Jun 2016
ERR1378291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066368
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA501.9819727E-631.49891930
GACCGTT608.193012E-626.24909629
TCGTATG608.193012E-626.24909643
GCGGTTC551.3679957E-424.5457638
CGTATGC551.3684313E-424.54461144
CGTTAAA551.3684313E-424.54461132
CCGTTAA551.3684313E-424.54461131
CTCGTAT651.524023E-524.22993742
AGACCGT651.524023E-524.22993728
TGCCGAG602.467922E-422.49922423
ATGCCGA706.9846335E-419.2850522
GGGGACG600.00651148918.74935330
CGGTTCA750.001110408117.9993829
GAGACCG750.001110408117.99938227
TCTCGTA750.001110408117.99938241
ATCTCGT800.001710592516.8752140
AGCGGTT800.001710592516.875217
GAGCGGT800.001710592516.875216
CCCGCCA1602.9572402E-715.471123
CGCCCGC1209.7228185E-515.0051131
Produced by FastQC (version 0.11.5)