FastQCFastQC Report
Mon 6 Jun 2016
ERR1378289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166926
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT300.00514824929.9988624
TCTCGTA453.4917975E-529.99885741
CGTATGC507.160346E-526.99897244
CTCGTAT507.160346E-526.99897242
AGACCGG608.194111E-626.24928
GCGGTTC551.3681762E-424.545578
GACCGGG551.3685742E-424.5445229
GAGCGGT602.4667452E-422.5010726
AGAGCGG602.4667452E-422.5010725
CCGGGAT654.239373E-420.76844231
CGGTTCA654.239373E-420.7684429
AAGAGCG706.971259E-419.2907664
AGCGGTT706.983333E-419.2858077
CGGCTAA1951.8189894E-1218.46083517
ACGCCCG952.8202205E-416.57831612
ACCGGGA850.00256584915.88174730
TCGTATG850.00256584915.88174743
CCGGCTA2151.5279511E-1015.69707616
GGGCGAC900.003746363815.0045723
GAGACCG900.00375491914.99942927
Produced by FastQC (version 0.11.5)