FastQCFastQC Report
Mon 6 Jun 2016
ERR1378288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156288
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT702.1827873E-1135.3577229
CGTATGC855.456968E-1231.76522444
CCGATTG300.00514903829.99789218
ACCGATG852.0372681E-1029.11812230
AGACCGA957.421477E-1026.05305728
AGAGCGG1052.3683242E-923.5697755
TCTCGTA1152.6557245E-1023.47864341
CTCGTAT1104.0527084E-922.50036842
CCGATGG1003.6437996E-822.50036631
GAGACCG1156.7684596E-921.52208927
TCGTATG1156.7684596E-921.52208943
GAGCGGT1201.1061275E-820.6235526
ATGCCGA1351.9645086E-920.00032622
ATCTCGT1251.7669663E-819.80032340
GCGGTTC1302.7745045E-819.0371278
CGGAAGA1354.2588E-818.3383921
AGCGGTT1354.2757165E-818.3320477
AATGCCG1354.2757165E-818.33204721
CGATGGT1305.781658E-717.30797632
AAGAGCG1501.420376E-716.502414
Produced by FastQC (version 0.11.5)