FastQCFastQC Report
Mon 6 Jun 2016
ERR1378287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008553
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG702.8861905E-828.92780512
GTCCGGT406.166568E-428.12425614
CTCGTAT507.1564165E-527.00062442
GACCGTA602.4676602E-422.49940529
TCTCGTA654.2370724E-420.76971241
CGTAGGT654.2384904E-420.7686832
GCGGTTC807.563359E-519.686988
CGTATGC706.9815724E-419.2861644
TCGTATG706.9815724E-419.2861643
CGCCCGG1051.4455745E-619.28520613
CCGGCTA1403.086825E-919.28520416
CCGTAGG706.983902E-419.28520431
CGGCTAA1302.7721399E-819.03795817
ACCGTAG750.00111029217.99952530
ATCTCGT901.8650608E-417.50040440
CCGAGAC1053.1125164E-517.14240525
AGACCGT850.002565325515.88193328
CACCCGG850.002565325515.88193313
AAGAGCG1004.1641042E-415.7511464
GAGCGGT1004.1641042E-415.7511466
Produced by FastQC (version 0.11.5)