FastQCFastQC Report
Mon 6 Jun 2016
ERR1378285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034615
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGT300.005147729.99919338
ACCGGGA654.238604E-420.76867330
AAAGCGC800.001709337116.8769955
CGGATCA800.001711549816.87373211
AGATCGC850.002560432515.8865343
ACTAGAC4400.013.80577919
GAACCCG1504.2727166E-513.49898621
GAGATCG1555.786502E-513.069852
ACGCCCA1250.002252526812.59905112
TAGACAG6500.012.46060221
ACCACGC2004.978747E-511.2491549
AGGCCGA1650.001432793810.90879842
ACGGTGG1450.00682023810.8628277
CTAGACA7050.010.85024720
ACTCGGG2108.1735794E-510.71399834
AACCCGG1903.8671622E-410.65760922
ACCCGGG1954.91465E-410.38433623
AACTAGA6500.010.38383518
TACTCGG1750.00233846510.28543933
CACGCCC1750.00233940410.28494111
Produced by FastQC (version 0.11.5)