FastQCFastQC Report
Mon 6 Jun 2016
ERR1378284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025561
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAC352.818536E-432.1460237
CTCGTAT352.8209563E-432.14132342
CGTATGC406.167407E-428.12365744
CGGTTCA507.1599825E-526.9987139
GAGACCG500.002265072222.49892827
TCTCGTA500.002265072222.49892841
TCGTATG500.002265072222.49892843
ACCGGAA550.0039373420.45357130
GCGGTTC550.0039373420.4535718
GACCGGA550.0039373420.45357129
AGACCGG550.0039373420.45357128
CGGAGGT1605.02737E-614.06182933
CCCGGCC1902.0617954E-613.02569515
CGCCACC1607.809706E-512.6581155
CGTGAGC2207.887611E-712.2763321
GAATCGC1506.45051E-411.99942812
GCGGAGG1902.9480263E-511.84154132
CACGCCC1400.005257359211.24946311
AGAATCG1600.00110815611.24946311
GGCGGAG2251.35955215E-510.999475531
Produced by FastQC (version 0.11.5)