FastQCFastQC Report
Mon 6 Jun 2016
ERR1378282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937241
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAG803.6379788E-1233.7495630
TCTCGTA901.2732926E-1129.99960941
ACGGTCC300.005147107429.99960941
CGTATGC1004.5474735E-1126.9996544
CTCGTAT1004.5474735E-1126.9996542
CGAAGCT1004.5474735E-1126.9996532
AGAGCGG1058.54925E-1125.7153225
GACCGAA1058.54925E-1125.71395129
CCGAGAC1058.54925E-1125.71395125
CGGCTAA1550.024.677117
GAGCGGT1101.5097612E-1024.5464466
TCGTATG1101.5097612E-1024.54513443
CCGGCTA1650.023.18151716
ATCTCGT1405.456968E-1222.49970640
AGCGGTT1204.5110937E-1022.4997067
CGGAAGA1358.367351E-1121.6698531
CCGAAGC1257.512426E-1021.59971831
ACGCCCG654.2378006E-420.76895912
CGGTTCA1301.2259989E-920.7689599
AATGCCG1301.2259989E-920.76895921
Produced by FastQC (version 0.11.5)