FastQCFastQC Report
Mon 6 Jun 2016
ERR1378281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944362
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT551.3681361E-424.54496442
GACCGTT551.3681361E-424.54496429
ATGCCGA551.3681361E-424.54496422
GCGGTTC651.5223637E-524.2328518
AGCGGTT702.7005399E-522.5019327
ACCGTTC602.467391E-422.49955230
GAGCGGT654.235003E-420.7710156
ATCTCGT807.562338E-519.68710540
CGGTTCA807.562338E-519.6871059
CGTTCCT807.562338E-519.68710532
TCTCGTA706.983145E-419.2853341
AGACCGT706.983145E-419.2853328
TCGTATG706.983145E-419.2853343
CATCTCG851.204337E-418.52904139
CTTCGTA750.001109384718.0015457
AATGCCG750.001110173317.9996421
CCGAGAC750.001110173317.9996425
AGAGCGG800.001709557516.876455
ATCGTGC952.8168404E-416.5803725
AAGAGCG850.002563241215.8837184
Produced by FastQC (version 0.11.5)