FastQCFastQC Report
Mon 6 Jun 2016
ERR1378280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217971
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGC903.2844946E-722.501975
CTCGTAT550.00393748320.45381742
CGGTGAT550.00393748320.45381718
GACCGCT600.006512050518.74933229
AGATCGT1153.440733E-617.6131293
GATCGTG1053.1059488E-517.1471734
CGCTCCT800.001711331716.874432
TCGTGCC1104.634404E-516.3650686
ATCTCGT900.003754981614.99946640
CCTTCGT1656.889317E-613.6403581
CGTGAGC2053.351306E-713.174591
TGAGCCG2959.640644E-1112.9656445
CGACAGA1651.0433435E-412.2722931
GCGACAG1300.00302248212.11495430
CAACGAA1558.4896665E-411.6134437
AACCCGG1650.001433214610.90870222
CCAACGA1450.0068205110.863026
ACCCGGG1450.006826756510.86168323
ACCCGGC1450.006826756510.86168314
GCTAGAC2108.177044E-510.71390318
Produced by FastQC (version 0.11.5)