FastQCFastQC Report
Mon 6 Jun 2016
ERR1378279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770614
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT504.8763468E-835.9992742
GACCGGA602.4176552E-729.99939529
CCGGAAC602.4176552E-729.99939531
AATGCCG702.8841896E-828.92798621
ACCGGAA654.8707443E-727.6917530
GCGGTTC654.8707443E-727.691758
GAGCGGT654.8707443E-727.691756
CGTATGC709.302512E-725.71376444
CCGAGAC709.302512E-725.71376425
CGGTTCA801.05304935E-725.311999
AGCGGTT801.05304935E-725.311997
CGGAACT751.6967333E-623.99951732
AGACCGG751.6967333E-623.99951728
ATGCCGA751.6967333E-623.99951722
ATCTCGT802.9731655E-622.49954640
ACGGGTT500.002264203322.49954613
CGGGAAC500.002264203322.49954635
TCTCGTA855.0295366E-621.17604341
TCGTATG855.0295366E-621.17604343
TGCCGAG855.0295366E-621.17604323
Produced by FastQC (version 0.11.5)