FastQCFastQC Report
Mon 6 Jun 2016
ERR1378278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099650
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTT602.4180554E-730.0010917
CTCGTAT602.4199835E-729.99836242
CGGGTAT709.311352E-725.71288332
CGTATGC709.311352E-725.71288344
CGGAAGA801.0468648E-725.3295481
CCGGGTA751.6983413E-623.9986931
GCGGTTC802.9747935E-622.4997968
ACCGGGT802.9759685E-622.49877430
TCTCGTA802.9759685E-622.49877441
TCGTATG802.9759685E-622.49877443
GAGCGGT855.0283124E-621.1782046
CGGTTCA855.0342587E-621.1753169
AGACCGG908.254607E-619.99890928
GAGACCG1051.4462075E-619.28466227
CCGAGAC1051.4462075E-619.28466225
AGAGCGG951.3148185E-518.948925
GACCGGG951.3163659E-518.94633529
ATCTCGT1002.0474648E-517.99901840
ATGCCGA1205.1753777E-616.87407922
AGCGCTC900.003752874415.0005467
Produced by FastQC (version 0.11.5)