FastQCFastQC Report
Mon 6 Jun 2016
ERR1378277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680081
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC406.8102963E-944.9977944
TCGTATG406.8102963E-944.9977943
CTCGTAT504.875983E-835.99823442
TCTCGTA551.1270458E-732.7256741
CGGATAA501.980803E-631.49845534
AGAGCGG602.4128894E-730.0051465
CCGCGGA554.1632393E-628.6349631
GCGGTTC654.870544E-727.6909528
GCGGATA608.188239E-626.2487133
CGCGGAT651.5231402E-524.22958432
ACCGCGG651.5231402E-524.22958430
ATCTCGT751.6966715E-623.99882340
CGCCTGG751.6966715E-623.99882313
TGCCGAG751.6966715E-623.99882323
AGCGGTT802.969262E-622.5022077
AGACCGC702.7019194E-522.49889828
CGGTTCA855.0293584E-621.1754329
AATCTCG855.0293584E-621.17543239
ATGCCGA855.0293584E-621.17543222
GAGCGGT908.230898E-620.0034316
Produced by FastQC (version 0.11.5)