FastQCFastQC Report
Mon 6 Jun 2016
ERR1378273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269836
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC708.1490725E-1032.14260544
CTCGTAT702.886918E-828.92834342
TCTCGTA801.0540498E-725.31230241
ACGCCCG702.7042686E-522.49893612
GCGGTTC903.2890057E-722.4989368
ATGCCGA903.2890057E-722.49893622
AGCGGTT955.535185E-721.3147837
CGTTTGG654.2399194E-420.76825131
GACCGTG1009.06055E-720.24904429
CGTATAA1051.4464676E-619.28480310
CGGTTCA1051.4464676E-619.2848039
AATGCCG1051.4464676E-619.28480321
AGACCGT1051.4464676E-619.28480328
GAGCGGT1102.2539807E-618.4111216
TCGTATG1102.2565273E-618.40894743
CGGAAGA1153.4352724E-617.6161861
CCGAGAC1153.4507957E-617.60786225
TCGTATA1205.176289E-616.8742039
AGAGCGG1257.619443E-616.2017865
GAGACCG1257.6308515E-616.19923427
Produced by FastQC (version 0.11.5)