FastQCFastQC Report
Mon 6 Jun 2016
ERR1378272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064848
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT356.247097E-638.5721142
CGTATGC401.5602704E-533.750644
GAGCGGT401.5602704E-533.75066
CGGTATG401.5602704E-533.750632
AAGAGCG507.1570495E-527.0004774
ACCGGTA551.3679458E-424.5458930
TCGTATG551.3679458E-424.5458943
GACCGGT602.467048E-422.50039929
CCGGTAT602.467048E-422.50039931
AGACCGG754.6015964E-521.00037228
TCTCGTA654.237442E-420.76959841
ACCGTGC654.237442E-420.7695982
ATCTCGT807.5613E-519.68784940
AGCGGTT706.9821754E-419.2860557
AGAGCGG706.9821754E-419.2860555
GAGACCG851.2041718E-418.5297427
GCGGTTC800.001711068316.8745068
ATGCCGA850.002565499215.88188922
ACCACGC2651.8189894E-1215.2825729
CGTTGGA2001.5723344E-814.6252585
Produced by FastQC (version 0.11.5)