FastQCFastQC Report
Mon 6 Jun 2016
ERR1378269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281188
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC602.4202745E-729.99869744
CTCGTAT851.8948049E-723.82249542
GCGGTTC851.8948049E-723.8224958
ACCGAGT851.8948049E-723.82249530
GAGCGGT802.972285E-622.5025376
AGCGGTT802.9753082E-622.49997
TATCTCG802.9753082E-622.499939
TCGTATG855.0348444E-621.1755543
GACCGAG1109.990981E-820.45365729
TCTCGTA1009.06035E-720.2491241
CGGTTCA1153.4507175E-617.6079319
AGACCGA1053.114032E-517.14211528
ATGCCGA1205.1761726E-616.87426822
CCGAGAC1104.639906E-516.36292625
TCGTATA1401.2555374E-616.0707329
AATGCCG1156.7859866E-515.65149421
ATCTTCG1654.1955172E-715.0016915
CTTCGTA1654.2010834E-714.9999357
CGCCACC2304.2382453E-1014.6755685
ATCTCGT1251.380698E-414.39993840
Produced by FastQC (version 0.11.5)